BLZF1 antibody (AA 10-59)

Catalog No. ABIN1534121

The Rabbit Polyclonal anti-BLZF1 antibody (ABIN1534121) specifically detects BLZF1 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for BLZF1 antibody (AA 10-59) (ABIN1534121)

Target: BLZF1 (Basic Leucine Zipper Nuclear Factor 1 (BLZF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLZF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BLZF1 Antibody

Binding Specificity: AA 10-59

Purpose: Golgin 45 Antibody

Specificity: Golgin 45 Polyclonal Antibody detects endogenous levels of Golgin 45 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for BLZF1

Target: BLZF1 (Basic Leucine Zipper Nuclear Factor 1 (BLZF1))

Alternative Name: BLZF1

Background:

Synonyms: BLZF1, JEM1, Golgin-45, Basic leucine zipper nuclear factor 1, JEM-1, p45 basic leucine-zipper nuclear factor

Background: caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.,function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.,induction:Up-regulated by retinoids.,subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi.,tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.,

Gene Name: BLZF1

Protein Name: Golgin-45

Molecular Weight: 44 kDa

Gene ID: 8548

OMIM: 608692

UniProt: Q9H2G9