TCFL5 antibody (AA 311-360)

Catalog No. ABIN1534189

The Rabbit Polyclonal anti-TCFL5 antibody is suitable to detect TCFL5 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for TCFL5 antibody (AA 311-360) (ABIN1534189)

Target: TCFL5 (Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCFL5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TCFL5 Antibody

Binding Specificity: AA 311-360

Purpose: TCFL5 Antibody

Specificity: TCFL5 Polyclonal Antibody detects endogenous levels of TCFL5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TCFL5. AA range:311-360

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TCFL5

Target: TCFL5 (Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5))

Alternative Name: TCFL5

Background:

Synonyms: TCFL5, CHA, E2BP1, Transcription factor-like 5 protein, Cha transcription factor, HPV-16 E2-binding protein 1, E2BP-1

Background: developmental stage:Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.,disease:Antibodies against TCFL5 are present in sera from patients with Chagas' disease, an autoimmune disease caused by Trypanosoma cruzi. Two different epitopes that mimic Trypanosoma cruzi antigens have been identified: R1 and R3 epitopes, which are recognized by T- and B-cells, respectively.,function:Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,tissue specificity:Isoform 3 is testis specific. Isoform 2 is pancreas specific.,

Gene Name: TCFL5

Protein Name: Transcription factor-like 5 protein

Molecular Weight: 48 kDa

Gene ID: 10732

OMIM: 604745

UniProt: Q9UL49