MED23 antibody (AA 1-50)

Catalog No. ABIN1534191

This Rabbit Polyclonal antibody specifically detects MED23 in ELISA, WB, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for MED23 antibody (AA 1-50) (ABIN1534191)

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED23 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MED23 Antibody

Binding Specificity: AA 1-50

Purpose: CRSP130 Antibody

Specificity: CRSP130 Polyclonal Antibody detects endogenous levels of CRSP130 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MED23. AA range:1-50

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MED23

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Alternative Name: MED23

Background:

Synonyms: MED23, ARC130, CRSP3, DRIP130, KIAA1216, SUR2, Mediator of RNA polymerase II transcription subunit 23, Activator-recruited cofactor 130 kDa component, ARC130, Cofactor required for Sp1 transcriptional activation subunit 3, CRSP complex subu

Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus.The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012],

Gene Name: MED23

Protein Name: Mediator of RNA polymerase II transcription subunit 23

Molecular Weight: 156 kDa

Gene ID: 9439

OMIM: 605042

UniProt: Q9ULK4

Pathways: Regulation of Lipid Metabolism by PPARalpha