14-3-3 gamma antibody (AA 51-100)

Catalog No. ABIN1534235

The Rabbit Polyclonal anti-14-3-3 gamma antibody (ABIN1534235) specifically detects 14-3-3 gamma in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for 14-3-3 gamma antibody (AA 51-100) (ABIN1534235)

Target: 14-3-3 gamma (YWHAG1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-14-3-3 gamma Antibody

Binding Specificity: AA 51-100

Purpose: 14-3-3 γ Antibody

Specificity: 14-3-3 γ Polyclonal Antibody detects endogenous levels of 14-3-3 γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 gamma. AA range:51-100

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Alternative Name: 14-3-3 gamma

Background:

Synonyms: YWHAG, 14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008],

Gene Name: YWHAG

Protein Name: 14-3-3 protein gamma

Molecular Weight: 28 kDa

Gene ID: 7532

OMIM: 605356

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase