CD3EAP antibody (AA 441-490)

Catalog No. ABIN1534844

The Rabbit Polyclonal anti-CD3EAP antibody (ABIN1534844) specifically detects CD3EAP in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CD3EAP antibody (AA 441-490) (ABIN1534844)

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3EAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD3EAP Antibody

Binding Specificity: AA 441-490

Purpose: CD3EAP Antibody

Specificity: CD3EAP Polyclonal Antibody detects endogenous levels of CD3EAP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CD3EAP. AA range:441-490

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CD3EAP

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Alternative Name: CD3EAP

Background:

Synonyms: CD3EAP, ASE1, CAST, PAF49, DNA-directed RNA polymerase I subunit RPA34, A34.5, Antisense to ERCC-1 protein, ASE-1, CD3-epsilon-associated protein, CAST, CD3E-associated protein, RNA polymerase I-associated factor PAF49

Background: caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.,function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.,miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function.,PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.,PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family.,subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.,

Gene Name: CD3EAP

Protein Name: DNA-directed RNA polymerase I subunit RPA34

Molecular Weight: 54 kDa

Gene ID: 10849

OMIM: 107325

UniProt: O15446