NAD-ME antibody (AA 201-250)

Catalog No. ABIN485674

This Rabbit Polyclonal antibody specifically detects NAD-ME in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NAD-ME antibody (AA 201-250) (ABIN485674)

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAD-ME antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NAD-ME Antibody

Binding Specificity: AA 201-250

Purpose: ME2 Antibody

Specificity: ME2 Polyclonal Antibody detects endogenous levels of ME2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ME2. AA range:201-250

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for NAD-ME

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Alternative Name: ME2

Background:

Synonyms: ME2, NAD-dependent malic enzyme, mitochondrial, NAD-ME, Malic enzyme 2

Background: This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009],

Gene Name: ME2

Protein Name: NAD-dependent malic enzyme mitochondrial

Molecular Weight: 65 kDa

Gene ID: 4200

OMIM: 154270

UniProt: P23368

Pathways: Production of Molecular Mediator of Immune Response