MT-ND1 antibody (AA 176-225)

Catalog No. ABIN1535071

The Rabbit Polyclonal anti-MT-ND1 antibody is suitable to detect MT-ND1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for MT-ND1 antibody (AA 176-225) (ABIN1535071)

Target: MT-ND1 (Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MT-ND1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MT-ND1 Antibody

Binding Specificity: AA 176-225

Purpose: ND1 Antibody

Specificity: ND1 Polyclonal Antibody detects endogenous levels of ND1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MT-ND1. AA range:176-225

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MT-ND1

Target: MT-ND1 (Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1))

Alternative Name: MT-ND1

Background:

Synonyms: MT-ND1, MTND1, NADH1, ND1, NADH-ubiquinone oxidoreductase chain 1, NADH dehydrogenase subunit 1

Background: catalytic activity:NADH + ubiquinone = NAD(+) + ubiquinol.,disease:Defects in MT-ND1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.,disease:Defects in MT-ND1 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.,disease:Defects in MT-ND1 may be associated with mitochondrial susceptibility to Alzheimer disease (AD) [MIM:502500].,disease:Defects in MT-ND1 may be associated with non-insulin-dependent diabetes mellitus (NIDDM).,function:Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.,similarity:Belongs to the complex I subunit 1 family.,

Gene Name: MT-ND1

Protein Name: NADH-ubiquinone oxidoreductase chain 1

Molecular Weight: 35 kDa

Gene ID: 4535

OMIM: 502500

UniProt: P03886