ZP4 antibody (AA 231-280)

Catalog No. ABIN1535494

The Rabbit Polyclonal anti-ZP4 antibody is suitable to detect ZP4 in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for ZP4 antibody (AA 231-280) (ABIN1535494)

Target: ZP4 (Zona Pellucida Glycoprotein 4 (ZP4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ZP4 Antibody

Binding Specificity: AA 231-280

Purpose: ZP4 Antibody

Specificity: ZP4 Polyclonal Antibody detects endogenous levels of ZP4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ZP4. AA range:231-280

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ZP4

Target: ZP4 (Zona Pellucida Glycoprotein 4 (ZP4))

Alternative Name: ZP4

Background:

Synonyms: ZP4, ZPB, Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B

Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been

Gene Name: ZP4

Protein Name: Zona pellucida sperm-binding protein 4

Molecular Weight: 59 kDa

Gene ID: 57829

UniProt: Q12836