Histone H1 antibody (acLys25)

Catalog No. ABIN1535513

The Rabbit Polyclonal anti-Histone H1 antibody (ABIN1535513) specifically detects Histone H1 in WB, IF, IHC and ELISA. The antibody is reactive with Human and Monkey samples.

Quick Overview for Histone H1 antibody (acLys25) (ABIN1535513)

Target: Histone H1

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Product Details anti-Histone H1 Antibody

Binding Specificity: AA 131-180, acLys25

Purpose: Acetyl Histone H1 (K25) Antibody

Specificity: Acetyl-Histone H1 (K25) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when acetylated at K25.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H1 around the acetylated site of Lys25. AA range:131-180

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Histone H1

Target: Histone H1

Background:

Synonyms: H1FOO, H1OO, OSH1, Histone H1oo, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1, H1K25AC

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Oct 2015],

Gene Name: H1FOO

Protein Name: Histone H1oo

Molecular Weight: 21 kDa

Gene ID: 132243

UniProt: Q8IZA3