VN2R1P antibody (AA 400-449)

Catalog No. ABIN1535664

This Rabbit Polyclonal antibody specifically detects VN2R1P in ELISA, WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for VN2R1P antibody (AA 400-449) (ABIN1535664)

Target: VN2R1P (Vomeronasal 2 Receptor 1 Pseudogene (VN2R1P))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VN2R1P antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-VN2R1P Antibody

Binding Specificity: AA 400-449

Purpose: VN2R1P Antibody

Specificity: VN2R1P Polyclonal Antibody detects endogenous levels of VN2R1P protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CASRL1. AA range:400-449

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for VN2R1P

Target: VN2R1P (Vomeronasal 2 Receptor 1 Pseudogene (VN2R1P))

Background:

Background: The CARL-1 monoclonal antibody reacts with human TWEAK, a type II transmembrane TNF superfamily member with high identity to TNF in its extracellular portion. TWEAK transcript is expressed broadly in many adult and fetal tissues, however, the staining of human peripheral blood mononuclear cells with monoclonal antibodies shows a more restricted pattern. While freshly isolated PBMCs do not express detectable levels of TWEAK on their surface, IFN-gamma-stimulated blood monocytes rapidly upregulate TWEAK surface expression. TWEAK is expressed as membrane bound and secreted forms. Interaction of TWEAK with its counter-receptor promotes secretion of IL-8, activation of NF-kappaB, proliferation of endothelial cells, and apoptosis in a number of human cell lines. Initially, DR3 was thought to be a receptor for TWEAK, but further studies have shown that TWEAK could induce apoptosis via receptors distinct from DR3. While TWEAK exhibits overlapping signaling functions to TNF, it is generally less effective in inducing apoptosis, giving rise to its name, TNF-like weak inducer of apoptosis. For detection of human TWEAK by sandwich ELISA, a combination of purified CARL-2 for capture and biotinylated CARL-1 for detection is recommended.

Gene Name: CASRL1

Protein Name: Putative calcium-sensing receptor-like 1

Molecular Weight: 83 kDa

Gene ID: 344760

UniProt: Q8NGV9