Aggrecan antibody (Cleaved-Asp369)

Catalog No. ABIN1536077

The Rabbit Polyclonal anti-Aggrecan antibody (ABIN1536077) specifically detects Aggrecan in WB, IHC, ELISA and IF. The antibody is reactive with Human and Rat samples.

Quick Overview for Aggrecan antibody (Cleaved-Asp369) (ABIN1536077)

Target: Aggrecan (ACAN)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Aggrecan Antibody

Binding Specificity: AA 320-369, Cleaved-Asp369

Purpose: Cleaved-Aggrecan (D369) Antibody

Specificity: Cleaved-Aggrecan (D369) Polyclonal Antibody detects endogenous levels of fragment of activated Aggrecan protein resulting from cleavage adjacent to D369.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Aggrecan. AA range:320-369

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan

Background:

Synonyms: ACAN, AGC1, CSPG1, MSK16, Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1

Background: This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008],

Gene Name: ACAN

Protein Name: Aggrecan core protein

Molecular Weight: 44 kDa

Gene ID: 176

OMIM: 155760

UniProt: P16112

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport