Coagulation Factor X antibody (Cleaved-Ile235)

Catalog No. ABIN486716

The Rabbit Polyclonal anti-Coagulation Factor X antibody is suitable to detect Coagulation Factor X in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Coagulation Factor X antibody (Cleaved-Ile235) (ABIN486716)

Target: Coagulation Factor X (F10)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Coagulation Factor X antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Coagulation Factor X Antibody

Binding Specificity: AA 216-265, Cleaved-Ile235

Purpose: Cleaved-Factor Xa activated HC (I235) Antibody

Specificity: Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody detects endogenous levels of fragment of activated Factor Xa activated HC protein resulting from cleavage adjacent to I235.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FA10. AA range:216-265

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Coagulation Factor X

Target: Coagulation Factor X (F10)

Alternative Name: FA10

Background:

Synonyms: F10, Coagulation factor X, Stuart factor, Stuart-Prower factor

Background: This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds, the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative sp

Gene Name: F10

Protein Name: Coagulation factor X

Molecular Weight: 30 kDa

Gene ID: 2159

OMIM: 227600

UniProt: P00742