MASP1 antibody (Cleaved-Arg448)

Catalog No. ABIN1536127

The Rabbit Polyclonal anti-MASP1 antibody is suitable to detect MASP1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for MASP1 antibody (Cleaved-Arg448) (ABIN1536127)

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MASP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MASP1 Antibody

Binding Specificity: AA 399-448, Cleaved-Arg448

Purpose: Cleaved-MASP-1 HC (R448) Antibody

Specificity: Cleaved-MASP-1 HC (R448) Polyclonal Antibody detects endogenous levels of fragment of activated MASP-1 HC protein resulting from cleavage adjacent to R448.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MASP1. AA range:399-448

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MASP1

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Alternative Name: MASP1

Background:

Synonyms: MASP1, CRARF, CRARF1, PRSS5, Mannan-binding lectin serine protease 1, Complement factor MASP-3, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, MASP-1, Mannose-binding protein-asso

Background: mannan binding lectin serine peptidase 1(MASP1) Homo sapiens This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[p

Gene Name: MASP1

Protein Name: Mannan-binding lectin serine protease 1

Molecular Weight: 47 kDa

Gene ID: 5648

OMIM: 600521

UniProt: P48740

Pathways: Complement System