Cytochrome C antibody
Quick Overview for Cytochrome C antibody (ABIN155090)
Target
See all Cytochrome C (CYCS) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- The cytochrome c antibody detects the 12 kDa cytochrome c.
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Purification
- Affinity purified
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Immunogen
- Bovine cytochrome c
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Application Notes
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Western Blot: 0.5-4 μg/mL. Mouse small intestine lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.2 mg/mL
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Buffer
- PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal
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Preservative
- Thimerosal (Merthiolate)
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Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- -20 °C/-80 °C
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Storage Comment
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
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- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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Alternative Name
- Cytochrome C
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Background
- Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.Synonyms: CYC, CYCS
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Gene ID
- 13063
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NCBI Accession
- NP_031834
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UniProt
- P62897
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Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Target
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