FLT1 antibody (Extracellular Domain) (Biotin)

Catalog No. ABIN155162

The Mouse Monoclonal anti-FLT1 antibody has been validated for WB, IP, IF and EIA. It is suitable to detect FLT1 in samples from Human and Mouse.

Quick Overview for FLT1 antibody (Extracellular Domain) (Biotin) (ABIN155162)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLT1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: FLTEWF

Product Details anti-FLT1 Antibody (Biotin)

Binding Specificity: Extracellular Domain

Specificity: The unconjugated monoclonal antibody will recognize recombinant and naturally occurring form of VEGFR-1/Flt-1.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Protein G Chromatography

Immunogen: Recombinant Human soluble extracellular Flt-1 protein (D5)

Isotype: IgG1

Application Details

Application Notes: ELISA (undiluted 1.5 - 8 μg/mL). Western blotting (1 - 10 μg/mL). Immunofluorescence. Immunoprecipitation (1 - 5 μg/mL lysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, 0.02 % Sodium Azide, 50X BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: VEGFR-1 / Flt-1

Background: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor

Gene ID: 2321

NCBI Accession: NP_001153392

UniProt: P17948

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals