CCR5 antibody (AA 1-22) (PE)

Catalog No. ABIN1558086

This anti-CCR5 antibody is a Mouse Monoclonal antibody detecting CCR5 in FACS. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for CCR5 antibody (AA 1-22) (PE) (ABIN1558086)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR5 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: T21-8

Product Details anti-CCR5 Antibody (PE)

Binding Specificity: AA 1-22

Purpose: Anti-Hu CD195 PE

Specificity: The mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: CCR5 peptide (Met1-Lys22) KLH conjugate

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CCR5 antibody (PE) (ABIN1558086)

Mascalchi, Lamort, Salomé, Dumas: "Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes." in: Biochemical and biophysical research communications, Vol. 417, Issue 1, pp. 409-13, (2012) (PubMed).

Monde, Maeda, Tanaka, Harada, Yusa: "Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5." in: The Journal of biological chemistry, Vol. 282, Issue 51, pp. 36923-32, (2007) (PubMed).

Hüttenrauch, Pollok-Kopp, Oppermann: "G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers." in: The Journal of biological chemistry, Vol. 280, Issue 45, pp. 37503-15, (2005) (PubMed).

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CD195

Background: C-C motif chemokine receptor 5,CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.,CKR5, CCR5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5

Gene ID: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size