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V5 Epitope Tag antibody (HRP)

WB, ELISA Host: Rabbit Polyclonal HRP
Catalog No. ABIN1573948
  • Target See all V5 Epitope Tag products
    V5 Epitope Tag
    Reactivity
    Please inquire
    Host
    • 63
    • 40
    • 7
    • 5
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 62
    • 46
    • 10
    Polyclonal
    Conjugate
    • 57
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This V5 Epitope Tag antibody is conjugated to HRP
    Application
    • 89
    • 46
    • 34
    • 34
    • 21
    • 19
    • 13
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 2
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    • 1
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    Western Blotting (WB), ELISA
    Sequence
    GKPIPNPLLG LDST
    Specificity
    Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
    Purification
    Immunoaffinity chromatography
    Immunogen
    V5 epitope tag peptide GKPIPNPLLGLDST conjugated - KLH
    Isotype
    IgG
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Dahl, Corydon, Ränkel, Nielsen, Füchtbauer, Knudsen: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, (2014) (PubMed).

    Tahir, Yang, Goltsov, Song, Ren, Wang, Chang, Thompson: "Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer." in: Cancer research, Vol. 73, Issue 6, pp. 1900-11, (2013) (PubMed).

    Zhang, Li, Jaiswal, Bayat, Xiong, Sandoval, Charng, David, Haueter, Yamamoto, Graham, Bellen: "The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 807-20, (2013) (PubMed).

  • Target
    V5 Epitope Tag
    Alternative Name
    V5-Tag (V5 Epitope Tag Products)
    Target Type
    Tag
    Background
    Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
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