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Avi-Tag antibody

ICC, FACS, IF, ELISA, WB Host: Mouse Monoclonal 1D11D10 unconjugated
Pubmed (4 references)
Catalog No. ABIN1574261
$160.67
Plus shipping costs $45.00
40 μg
Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target See all Avi-Tag products
    Avi-Tag
    Reactivity
    Please inquire
    Host
    • 5
    • 1
    Mouse
    Clonality
    • 5
    • 1
    Monoclonal
    Conjugate
    • 4
    • 1
    • 1
    This Avi-Tag antibody is un-conjugated
    Application
    Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Western Blotting (WB)
    Sequence
    GLNDIFEAQKIEWHE
    Specificity
    Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.
    Cross-Reactivity (Details)
    Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.
    Purification
    Protein A affinity column
    Immunogen
    Avi peptide conjugated - KLH
    Clone
    1D11D10
    Isotype
    IgG2a
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Western blot: 0.2-1 µg/mL FACS: 1-3 µg for 1 x 106 cells ICC/IF: 1-3 µg/mL ELISA: 0.02-0.05 µg/mL
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % sodium azide.
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Nogal, McCoy, van Gils, Cottrell, Voss, Andrabi, Pauthner, Liang, Messmer, Nedellec, Shin, Turner, Ozorowski, Sanders, Burton, Ward: "HIV envelope trimer-elicited autologous neutralizing antibodies bind a region overlapping the N332 glycan supersite." in: Science advances, Vol. 6, Issue 23, pp. eaba0512, (2020) (PubMed).

    Sheridan, Fong, DAlessandro, Bentley: "Widespread Backtracking by RNA Pol II Is a Major Effector of Gene Activation, 5' Pause Release, Termination, and Transcription Elongation Rate." in: Molecular cell, Vol. 73, Issue 1, pp. 107-118.e4, (2019) (PubMed).

    Hill, Martinko, Nguyen, Wells: "Human antibody-based chemically induced dimerizers for cell therapeutic applications." in: Nature chemical biology, Vol. 14, Issue 2, pp. 112-117, (2019) (PubMed).

    Iovino, Orihuela, Moorlag, Molema, Bijlsma: "Interactions between blood-borne Streptococcus pneumoniae and the blood-brain barrier preceding meningitis." in: PLoS ONE, Vol. 8, Issue 7, pp. e68408, (2014) (PubMed).

  • Target
    Avi-Tag
    Alternative Name
    Avi Tag (Avi-Tag Products)
    Target Type
    Tag
    Background
    Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.
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