SAG antibody

Catalog No. ABIN1580413

This Mouse Monoclonal antibody specifically detects SAG in WB, IHC, ICC and IF. It exhibits reactivity toward Human, Mouse, Rat, Cow and Pig.

Quick Overview for SAG antibody (ABIN1580413)

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S128

Product Details anti-SAG Antibody

Purification: affinity purified antibody

Isotype: IgG1

Application Details

Application Notes: Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal homogenate. The arrestin protein runs at about ~48 kDa on SDS-PAGE gels.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for SAG

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Alternative Name: Arrestin-1/ S-antigen

Background: The arrestin proteins are a family of regulators of cell signaling of G protein-coupled receptors (GPCR). Visual arrestin was first discovered as a result of the experimental model of human uveitis, an autoimmune disease of the eye. In this model, called experimental allergic uveitis, animals were injected with extracts made from the retina of the same species mixed with Freund's complete adjuvant. The animals mounted a strong immune response to the extract, and the antibody response was used to identify several immunogenic retinal proteins. One of these was called S-antigen, for soluble antigen. The protein was found to be abundant in retina, about 48 kDa in molecular weight, and localized in the outer segments of the photoreceptors. Several years later, Hermann K�hn and colleagues discovered that this protein binds to phosphorylated rhodopsin and prevents this protein from activating transducin. Transducin is a typical heterotrimeric G protein, composed of alpha, and beta,gamma, subunits. Rhodopsin phosphorylation is mediated by Rhodopsin kinase (a.k.a. GRK1), the prototypic member of a family of GPCR kinases. Since the S-antigen protein arrested the activity of rhodopsin it was renamed arrestin, and became the prototypic member of the arrestin protein family. Subsequently, Robert

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling