SF3B4 antibody

Catalog No. ABIN1580459

The Mouse Monoclonal anti-SF3B4 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect SF3B4 in samples from Human, Mouse, Rat, Cow, Pig and Mammalian.

Quick Overview for SF3B4 antibody (ABIN1580459)

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Reactivity: Human, Mouse, Rat, Cow, Pig, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This SF3B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3A1

Product Details anti-SF3B4 Antibody

Purification: affinity purified antibody

Isotype: IgG2b

Application Details

Application Notes: The antibody is affinity purified antibody diluted to 1mg/ml in phosphate buffered saline at a volume of 100 μl. The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000 or lower.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for SF3B4

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Background: SF3B4, also known as SAP49, is a ubiquitously expressed splicing factor found in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells. It was originally identified by as the protein most efficiently UV cross-linked to the A, B and C spliceosomal complexes. The protein contains two of the highly conserved RRM type RNA recognition motifs, each corresponding to a small approx. 70 amino acid structure, consisting of 4 beta strands and two alpha-helices. Proteins containing these proteins are believed to have a role in the regulation of mRNA splicing. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49 kDa. This protein is known as splicing factor 3b, subunit 4, 49 kDa SAP49, spliceosome-associated protein (U2 snRNP), Hsh49 and MGC108282. Antibodies to this protein are good markers of nuclei. The HGNC name for this protein is SF3B4.