TIE1 antibody

Catalog No. ABIN1589693

Product Details anti-TIE1 Antibody

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: TIE-1 antibody

Specificity: Recombinant human soluble TIE-2

Cross-Reactivity (Details): The monoclonal antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments and Western blotting.

Characteristics: Chromosomal location: 1p34-p33
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-1 as the immunizing antigen.

Purification: Mouse IgG1 antibody (#6F12) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Immunogen: Recombinant human soluble TIE-1

Clone: 6F12

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 1-15 μg/mL, Western blotting: Use at 1-2 μg/mL, FACS analysis and cell sorting: Test under progress.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE-1 (TIE1 Products)

Background: TIE1, TIE, JTK14,Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). It is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2.

Gene ID: 7075

NCBI Accession: NM_005424, NP_005415

UniProt: P35590

Pathways: RTK Signaling