FLT1 antibody (Biotin)

Catalog No. ABIN1589706

This anti-FLT1 antibody is a Mouse Monoclonal antibody detecting FLT1 in WB, IHC, ELISA and FACS. Suitable for Human.

Quick Overview for FLT1 antibody (Biotin) (ABIN1589706)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLT1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: EWC

Product Details anti-FLT1 Antibody (Biotin)

Specificity: Recombinant human soluble extracellular Flt-1 (D5)

Cross-Reactivity: Mouse (Murine)

Characteristics: Chromosomal location: 13q12
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular Flt-1 (D5) as the immunizing antigen. The monoclonal antibody will detect native and denatured human VEGFR-1/Flt-1 in ELISA experiments and on the surface of different human cell types. The antibody will also detect mouse Flt-1 (e.g. natural occurring soluble mouse Flt-1).

Purification: Mouse IgG1 antibody (clone EWC) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Isotype: IgG1

Application Details

Application Notes: ELISA: use at 1–10 µg/mL, Western blotting: use at 2–5 µg/mL, FC: use at 2-10 µg/mL

Comment:

Monoclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Centrifuge vial prior to opening.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: VEGFR-1/Flt-1

Background: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
Synonyms: vascular endothelial growth factor receptor-1, FLT1, VEGF receptor 1,

NCBI Accession: NM_001159920, NP_001153392

UniProt: P17948

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals