KRIT1 antibody

Catalog No. ABIN1589846

Product Details anti-KRIT1 Antibody

Target: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Recombinant human CCM1

Characteristics: Chromosomal location: 7q21.2

Isotype: IgG

Application Details

Application Notes: Western Blot: use 1-5 µg/mL

Comment:

Rabbit IG Polyclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: 0.5X PBS, pH 7.2

Handling Advice: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for KRIT1

Target: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Alternative Name: CCM-1 (KRIT1 Products)

Synonyms: CAM antibody, CCM1 antibody, 2010007K12Rik antibody, A630036P20Rik antibody, AA432855 antibody, AI450393 antibody, AI643869 antibody, BB155247 antibody, BB235701 antibody, Ccm1 antibody, RGD1305929 antibody, krit1 antibody, fb36f07 antibody, san antibody, santa antibody, wu:fb36f07 antibody, zgc:63585 antibody, KRIT1, ankyrin repeat containing antibody, KRIT1 antibody, Krit1 antibody, krit1 antibody

Background: Cerebral cavernous malformations (CCM) are frequent vascular abnormalities caused by mutations in one of the CCM genes. CCM-1 (also known as KRIT1) stabilizes endothelial junctions and is essential for vascular morphogenesis in mouse embryos. However, cellular functions of CCM-1 during the early steps of the CCM pathogenesis remain unknown. It was shown that CCM-1 represents an antiangiogenic protein to keep the human endothelium quiescent. CCM-1 inhibits endothelial proliferation, apoptosis, migration, lumen formation, and sprouting angiogenesis in primary human endothelial cells. CCM-1 strongly induces DLL4-NOTCH signaling, which promotes AKT phosphorylation but reduces phosphorylation of the mitogen-activated protein kinase ERK. Consistently, blocking of NOTCH activity alleviates CCM-1 effects. ERK phosphorylation is increased in human CCM lesions. Transplantation of CCM-1-silenced human endothelial cells into SCID mice recapitulates hallmarks of the CCM pathology and serves as a unique CCM model system.
Synonyms: CCM-1, Cerebral cavernous malformations protein 1, KRIT1, KRIT1, ankyrin repeat containing, CAM

Gene ID: 3

NCBI Accession: NP_004903, NM_004912

UniProt: O00522

Pathways: Cell RedoxHomeostasis