FLT4 antibody

Catalog No. ABIN1589862

This anti-FLT4 antibody is a Rat Monoclonal antibody detecting FLT4 in WB. Suitable for Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for FLT4 antibody (ABIN1589862)

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FLT4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 7A3

Product Details anti-FLT4 Antibody

Specificity: Recombinant mouse soluble FLT-4

Characteristics: Chromosomal location: 11 A5-B1, 11 25.0 cM
The hybridoma was generated after fusion with cells from rat immunized with purified, insect cell-derived recombinant mouse soluble FLT-4 (120 kDa) as the immunizing antigen.

Purification: Rat IgG1 antibody from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Application Details

Application Notes: Western Blot: use 1-5 µg/mL.

Comment:

Monoclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Expiry Date: 6 months

References for anti-FLT4 antibody (ABIN1589862)

Tempfer, Kaser-Eichberger, Korntner, Lehner, Kunkel, Traweger, Trost, Strohmaier, Bogner, Runge, Bruckner, Krefft, Heindl, Reitsamer, Schrödl: "Presence of lymphatics in a rat tendon lesion model." in: Histochemistry and cell biology, Vol. 143, Issue 4, pp. 411-9, (2015) (PubMed).

Target Details for FLT4

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Alternative Name: VEGFR-3/FLT-4

Background: VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.
Synonyms: vascular endothelial growth factor receptor-3, Flt4, Chy, Flt-4, VEGFR3, VEGFR-3,

NCBI Accession: NP_032055, NM_008029

UniProt: P35917

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling