LIF antibody

Catalog No. ABIN1589881

This Rabbit Polyclonal antibody specifically detects LIF in WB. It exhibits reactivity toward Human.

Quick Overview for LIF antibody (ABIN1589881)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIF Antibody

Purpose: LIF antibody

Specificity: Recombinant human LIF

Characteristics: Chromosomal location: 22q12.2
Produced from sera of rabbits immunized with highly pure recombinant human Leukemina Inhibitory Factor (LIF) [Ser23 - Phe202, produced in E. coli].

Purification: Anti-human LIF was purified by affinity chromatography with immobilized Protein A.

Immunogen: Recombinant human LIF (ABIN1589656)

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 2-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: LIF, CDF, DIA, HILDA, MLPLI,Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78 % identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF.

Gene ID: 3976

NCBI Accession: NM_002309, NP_002300

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding