FYB antibody (Internal Region)

Catalog No. ABIN1589991

This anti-FYB antibody is a Goat Polyclonal antibody detecting FYB in WB and ELISA. Suitable for Human.

Quick Overview for FYB antibody (Internal Region) (ABIN1589991)

Target: FYB (FYN-Binding Protein (FYB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FYB Antibody

Binding Specificity: Internal Region

Purpose: FYB

Sequence: SEEKPDKEPK P

Specificity: This antibody is expected to recognise all reported isoforms (NP_001456.3, NP_955367.1, NP_001230022.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in lysates of cell line MOLT4 (calculated MW of 90.6 kDa according to NP_001456.3). Recommended concentration: 0.2-0.6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FYB

Target: FYB (FYN-Binding Protein (FYB))

Alternative Name: FYB

Background: FYB, FYN binding protein (FYB-120/130) , ADAP, PRO0823, SLAP-130, FYN-binding protein (FYB-120/130), adhesion and degranulation-promoting adaptor protein

Gene ID: 2533

NCBI Accession: NP_001456, NP_955367, NP_001230022

Pathways: TCR Signaling, Protein targeting to Nucleus