EEF1E1 antibody (Internal Region)

Catalog No. ABIN1589993

The Goat Polyclonal anti-EEF1E1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect EEF1E1 in samples from Human and Pig.

Quick Overview for EEF1E1 antibody (Internal Region) (ABIN1589993)

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This EEF1E1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-EEF1E1 Antibody

Binding Specificity: Internal Region

Purpose: EEF1E1 / AIMP3 / p18

Sequence: SKGNKYSAQG ERQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_004271.1, NP_001129122.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.

Western Blot: Approx 19 kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8 kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8 kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3 μg/mL

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EEF1E1

Target: EEF1E1 (Eukaryotic Translation Elongation Factor 1 epsilon 1 (EEF1E1))

Alternative Name: EEF1E1

Background: EEF1E1, eukaryotic translation elongation factor 1 epsilon 1, AIMP3, P18, ARS-interacting multifunctional protein 3, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, eukaryotic translation elongation factor 1 epsilon-1

Gene ID: 9521

NCBI Accession: NP_004271, NP_001129122

Pathways: Positive Regulation of Response to DNA Damage Stimulus