MAPKAP Kinase 2 antibody (Internal Region)

Catalog No. ABIN1590006

This anti-MAPKAP Kinase 2 antibody is a Goat Polyclonal antibody detecting MAPKAP Kinase 2 in WB, IHC, ELISA and IP. Suitable for Human and Mouse.

Quick Overview for MAPKAP Kinase 2 antibody (Internal Region) (ABIN1590006)

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPKAP Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-MAPKAP Kinase 2 Antibody

Binding Specificity: Internal Region

Purpose: MK2 / MAPKAPK2

Sequence: SRVLKEDKER WED

Specificity: This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45 kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0 kDa accord

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Alternative Name: MAPKAPK2

Background: MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2

Gene ID: 9261, 17164, 289014

NCBI Accession: NP_004750, NP_116584

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades