MAPK6 antibody (Internal Region)

Catalog No. ABIN1590009

The Goat Polyclonal anti-MAPK6 antibody (ABIN1590009) specifically detects MAPK6 in WB, ELISA, IHC and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for MAPK6 antibody (Internal Region) (ABIN1590009)

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Grade: Verified

Product Details anti-MAPK6 Antibody

Binding Specificity: Internal Region

Purpose: ERK3 / MAPK6

Sequence: HSPVGSPLKS IQ

Specificity: Reported variants represent identical protein (NP_056621.4, NP_081694.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

Western Blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: Mapk6

Background: MAPK6, mitogen-activated protein kinase 6, ERK3, HsT17250, PRKM6, p97MAPK, ERK-3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activate

Gene ID: 5597, 50772, 58840

NCBI Accession: NP_002739

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C