MAPKAP Kinase 5 antibody (Internal Region)

Catalog No. ABIN1590011

The Goat Polyclonal anti-MAPKAP Kinase 5 antibody is suitable to detect MAPKAP Kinase 5 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IP.

Quick Overview for MAPKAP Kinase 5 antibody (Internal Region) (ABIN1590011)

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPKAP Kinase 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Grade: Verified

Product Details anti-MAPKAP Kinase 5 Antibody

Binding Specificity: Internal Region

Purpose: MK5 / MAPKAPK5

Sequence: STEALDNVLP SAQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8 μg/mL.

Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemi

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAPKAP Kinase 5

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Alternative Name: Mapkapk5

Background: MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase

Gene ID: 8550, 17165, 498183

NCBI Accession: NP_003659, NP_620777

Pathways: MAPK Signaling