MAPKAP Kinase 5 antibody (AA 192-206)
Quick Overview for MAPKAP Kinase 5 antibody (AA 192-206) (ABIN1590013)
Target
See all MAPKAP Kinase 5 (MAPKAPK5) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- AA 192-206
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Purpose
- MK5 / MAPKAPK5 (aa192-206)
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Sequence
- PQVLEAQRRH QKEKS
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Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotype
- IgG
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Application Notes
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Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8 μg/mL.
Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Bioche
Peptide ELISA: antibody detection limit dilution 1:64000.
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Comment
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Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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Alternative Name
- Mapkapk5
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Background
- MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase
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Gene ID
- 8550, 17165, 498183
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NCBI Accession
- NP_003659, NP_620777
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Pathways
- MAPK Signaling
Target
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