ZNRF1 antibody (Internal Region)

Catalog No. ABIN1590014

The Goat Polyclonal anti-ZNRF1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect ZNRF1 in samples from Human and Mouse.

Quick Overview for ZNRF1 antibody (Internal Region) (ABIN1590014)

Target: ZNRF1 (Zinc and Ring Finger Protein 1 (ZNRF1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ZNRF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ZNRF1 Antibody

Binding Specificity: Internal Region

Purpose: ZNRF1 (mouse)

Sequence: SDSTYAHGNG YQET

Specificity: This antibody is expected to recognize reported isoforms a and b (NP_573469.1, NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10 μg/mL. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan. Paraffin embedded Human

Western Blot: Approx 28 kDa band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8 kDa according to NP_573469.1. Recomme

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ZNRF1

Target: ZNRF1 (Zinc and Ring Finger Protein 1 (ZNRF1))

Alternative Name: Znrf1

Background: Znrf1, zinc and ring finger 1, B830022L21Rik, MGC101991, Rnf42, Zrfp1, nin283, E3 ubiquitin-protein ligase ZNRF1, nerve injury-induced gene 283 protein, peripheral nerve injury protein nin283, ring finger protein 42, zinc ring finger protein 1, zinc/RING

Gene ID: 84937, 170737, 690769

NCBI Accession: NP_573469, NP_001162093