BCAR1 antibody (Internal Region)

Catalog No. ABIN1590037

This Goat Polyclonal antibody specifically detects BCAR1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for BCAR1 antibody (Internal Region) (ABIN1590037)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-BCAR1 Antibody

Binding Specificity: Internal Region

Purpose: BCAR1

Sequence: KQTSTFSKQT PHH

Specificity: This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9 kDa according to NP_001164185.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1

Background: BCAR1, breast cancer anti-estrogen resistance 1, CAS, CAS1, CASS1, CRKAS, P130Cas, Cas scaffolding protein family member 1, Crk-associated substrate p130Cas, breast cancer anti-estrogen resistance protein 1

Gene ID: 9564, 12927, 25414

NCBI Accession: NP_001164185, NP_001164186, NP_001164187, NP_001164188, NP_001164189, NP_055382, NP_001164190, NP_001164191, NP_001164192

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling