Caspase 6 antibody (Internal Region)

Catalog No. ABIN1590060

The Goat Polyclonal anti-Caspase 6 antibody (ABIN1590060) specifically detects Caspase 6 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for Caspase 6 antibody (Internal Region) (ABIN1590060)

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Caspase 6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Caspase 6 Antibody

Binding Specificity: Internal Region

Purpose: Caspase 6 (alpha)

Sequence: NRKVSQRRVD FCKDP

Specificity: This antibody is expected to recognize reproted isoform alpha (NP_001217.2) only. The immunizing peptide overlaps phosphorylated serine 257 and therefore this antibody is not expected to recognize the phosphorylated protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 33 kDa band observed in Human Colon lysates (calculated MW of 33.3 kDa according to NP_001217.2). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Caspase 6

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Alternative Name: CASP6

Background: CASP6, caspase 6, apoptosis-related cysteine peptidase, MCH2, apoptotic protease MCH-2, caspase 6, apoptosis-related cysteine protease, caspase-6

Gene ID: 839, 12368, 83584

NCBI Accession: NP_001217, NP_116787

Pathways: Apoptosis, Caspase Cascade in Apoptosis