MMP14 antibody (Internal Region)

Catalog No. ABIN1590061

This Goat Polyclonal antibody specifically detects MMP14 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for MMP14 antibody (Internal Region) (ABIN1590061)

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MMP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-MMP14 Antibody

Binding Specificity: Internal Region

Purpose: MMP14

Sequence: ESATPLRFRE VP

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell lines NIH3T3, A549 and HeLa (calculated MW of 65.9 kDa according to NP_004986.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MMP14

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name: MMP14

Background: MMP14, matrix metallopeptidase 14 (membrane-inserted), 1, MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, matrix metalloproteinase 14 (membrane-inserted), matrix metalloproteinase-14, membrane type 1 metalloprotease, membrane-type matrix metall

Gene ID: 4323, 17387, 81707

NCBI Accession: NP_004986

Pathways: Autophagy