PKC beta antibody (Internal Region)

Catalog No. ABIN1590084

This anti-PKC beta antibody is a Goat Polyclonal antibody detecting PKC beta in WB, ELISA and IHC. Suitable for Human and Rat.

Quick Overview for PKC beta antibody (Internal Region) (ABIN1590084)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PKC beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PKC beta Antibody

Binding Specificity: Internal Region

Purpose: PRKCB

Sequence: DKEFTRQPVE LT

Specificity: This antibody is expected to recognize isoform 1 (NP_997700.1) only.

Cross-Reactivity: Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9 kDa according to NP_997700.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PRKCB

Background: PRKCB, protein kinase C, beta, MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, OTTHUMP00000162377, OTTHUMP00000162378, PKC-B, protein kinase C beta type, protein kinase C, beta 1 polypeptide

Gene ID: 5579

NCBI Accession: NP_997700

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling