SYVN1 antibody (Internal Region)

Catalog No. ABIN1590085

This Goat Polyclonal antibody specifically detects SYVN1 in WB, ELISA, FACS and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for SYVN1 antibody (Internal Region) (ABIN1590085)

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SYVN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-SYVN1 Antibody

Binding Specificity: Internal Region

Purpose: SYVN1

Sequence: HERQHLEARL QS

Specificity: This antibody is expected to recognize isoforms a and b (NP_115807.1, NP_757385.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Cerebellum and Rat Lung lysates (calculated MW of 67.6 kDa according to Human NP_115807.1 and 66.9 kDa according to Rat NP_001094209.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at roo

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and endoplasmic reticulum of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended c

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SYVN1

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Alternative Name: SYVN1

Background: SYVN1, synovial apoptosis inhibitor 1, synoviolin, HRD1, KIAA1810, MGC40372, synoviolin 1

Gene ID: 84447, 361712

NCBI Accession: NP_115807, NP_757385

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling