RPA1 antibody (AA 323-337)

Catalog No. ABIN1590115

This Goat Polyclonal antibody specifically detects RPA1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for RPA1 antibody (AA 323-337) (ABIN1590115)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RPA1 Antibody

Binding Specificity: AA 323-337

Purpose: RPA1 / RPA70 (aa323-337)

Sequence: CKSYEDATKI TVRSN

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70+80 kDa band observed in lysates of cell line HeLa (calculated MW of 68.1 kDa according to NP_002936.1). The upper band likely represents the acetylated protein. A 75 kDa band was observed in several Mouse cell lines and in lysates of

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA1

Background: RPA1, replication protein A1, 70 kDa, HSSB, MST075, REPA1, RF-A, RP-A, RPA70, MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, single-stranded DNA-binding protein

Gene ID: 6117

NCBI Accession: NP_002936

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA