CD44 antibody (Internal Region)

Catalog No. ABIN1590130

This Goat Polyclonal antibody specifically detects CD44 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD44 antibody (Internal Region) (ABIN1590130)

Target: CD44

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CD44 Antibody

Binding Specificity: Internal Region

Purpose: CD44

Sequence: NRDGTRYVQK GEYRT

Specificity: This antibody is expected to recognize reported isoforms 1, 2, 3, 4, 6, 7, 8 (NP_000601.3, NP_001001389.1, NP_001001390.1, NP_001001391.1, NP_001189484.1, NP_001189485.1, NP_001189486.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 75-82 kDa band observed in lysates of cell line U-2 OS (calculated MW of 81.5 kDa according to NP_000601.3 and 76.6 according to NP_001001389.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: CD44, CD44 Molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglyc

Gene ID: 960

NCBI Accession: NP_000601, NP_001001389, NP_001001390, NP_001001391, NP_001001392, NP_001189484, NP_001189485, NP_001189486

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling