CPB1 antibody (Biotin)

Catalog No. ABIN1607707

Product Details anti-CPB1 Antibody (Biotin)

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carboxypeptidase B from other sources may occur but have not been specifically determined.

Purity: Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].

Endotoxin Level: Low Endotoxin : No

Immunogen: Carboxypeptidase B [Porcine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-CARBOXYPEPTIDASE B (RABBIT) Antibody has been assayed against 1.0 µg of Carboxypeptidase B in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B (CPB1 Products)

Synonyms: wu:fb60d07 antibody, wu:fb69b08 antibody, CPB1 antibody, pCPB antibody, CPB antibody, PASP antibody, PCPB antibody, Cpb antibody, 0910001A18Rik antibody, 1810063F02Rik antibody, 2210008M23Rik antibody, AI504870 antibody, carboxypeptidase B1 (tissue) antibody, carboxypeptidase B1 antibody, carboxypeptidase B1 S homeolog antibody, carboxypeptidase B antibody, cpb1 antibody, CPB1 antibody, cpb1.S antibody, CpipJ_CPIJ010799 antibody, CpipJ_CPIJ010801 antibody, VDBG_02663 antibody, LOC100562609 antibody, Cpb1 antibody

Background: A carboxypeptidase (EC number 3.4.16 - 3.4.18) is a protease enzyme that hydrolyzes (cleaves) a peptide bond at the carboxy-terminal (C-terminal) end of a protein or peptide. (Contrast with an aminopeptidase, which cleaves peptide bonds at the other end of the protein.) Humans, animals, and plants contain several types of carboxypeptidases that have diverse functions ranging from catabolism to protein maturation. Carboxypeptidase B is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid. Synonyms: CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody

Gene ID: 397341

UniProt: P09955