CA2 antibody (Biotin)

Catalog No. ABIN1607715

Product Details anti-CA2 Antibody (Biotin)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carbonic Anhydrase II from other sources may occur but has not been specifically determined.

Purity: Anti-Carbonic Anyhdrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes].

Endotoxin Level: Low Endotoxin : No

Immunogen: Carbonic Anhydrase II [Human Erythrocytes]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Carbonic Anyhdrase II has been assayed against 1.0 µg of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20.000 - 1:100.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase II (CA2 Products)

Synonyms: CG6906 antibody, CT21171 antibody, Dmel\\CG6906 antibody, CA-2 antibody, caII antibody, car2 antibody, ca ii antibody, ca-ii antibody, BETA CA2 antibody, BETA CARBONIC ANHYDRASE 2 antibody, CA18 antibody, CARBONIC ANHYDRASE 18 antibody, CARBONIC ANHYDRASE 2 antibody, T9L3.40 antibody, T9L3_40 antibody, carbonic anhydrase 2 antibody, LOC100135827 antibody, AI131712 antibody, CAII antibody, Ca2 antibody, Car-2 antibody, Ltw-5 antibody, Lvtw-5 antibody, CA-II antibody, CAC antibody, Car2 antibody, CAR3 antibody, Carbonic anhydrase 2 antibody, carbonic anhydrase 2 L homeolog antibody, carbonic anhydrase 2 antibody, carbonic anhydrase 1 antibody, carbonic anhydrase II antibody, carbonic anhydrase, alpha type, periplasmic antibody, CAH2 antibody, ca2.L antibody, ca2 antibody, CA2 antibody, HCAG_04637 antibody, LOC100135827 antibody, LOC100635510 antibody, Car2 antibody, Ca2 antibody, LOC100154873 antibody, LOC101113341 antibody

Background: Anti-Carbonic Anhydrase II antibody is necessary for bone resorption and osteoclast differentiation and is involved in the reversible hydration of carbon dioxide. It plays a role in the modulating of fluid secretion into the anterior chamber of the eye. Belonging to the alpha-carbonic anhydrase family, Anti-Carbonic Anhydrase II antibody is ideal for investigators involved in the areas of Cardiovascular and Cell Biology research. Synonyms: Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody

Gene ID: 760

UniProt: P00918