Catalase antibody (Biotin)

Catalog No. ABIN1607783

Supplier Product No.: 200-4651-0100

This anti-Catalase antibody is a Rabbit Polyclonal antibody detecting Catalase in WB and ELISA. Suitable for Cow.

Quick Overview for Catalase antibody (Biotin) (ABIN1607783)

Target: Catalase (CAT)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Catalase Antibody (Biotin)

Supplier Product No.: 200-4651-0100

Supplier: Rockland

Purpose: Catalase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver].

Characteristics: Synonyms: rabbit anti-Catalase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

Purification: Anti-Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Catalase [Bovine Liver]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Catalase Biotin Conjugated Antibody has been tested by ELISA and western blot. This antibody is assayed against 1.0 μg of Catalase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500- 1:2,500

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: CAT

Background: Background: Anti-Catalase antibody serves to protect cells of aerobically respiring organisms from the negative effects of hydrogen peroxide. Additionally Catalase promotes cell growth including T-cells, B-cells, myeloid leukemia, melanoma, mastocytoma, and normal and transformed fibroblast cells. Belonging to the catalase family, Anti-Catalase antibody is ideal for researchers invested in Metabolism, Cancer , Cardiovascular , Signal Transduction, and Tags & Cell Marker research.

Gene ID: 531682

UniProt: P00432

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism