Urease antibody (Biotin)

Catalog No. ABIN1607815

Supplier Product No.: 200-4682-0100

The Rabbit Polyclonal anti-Urease antibody has been validated for WB, ELISA and IP. It is suitable to detect Urease in samples from Jack Bean.

Quick Overview for Urease antibody (Biotin) (ABIN1607815)

Target: Urease (URE)

Reactivity: Jack Bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urease antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Urease Antibody (Biotin)

Supplier Product No.: 200-4682-0100

Supplier: Rockland

Purpose: Urease Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

Characteristics: Synonyms: rabbit anti-Urease Antibody biotin Conjugation, biotin Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Purification: Anti-Urease antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Urease [Jack Bean]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Urease biotin conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Urease in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Urease

Target: Urease (URE)

Alternative Name: Urease

Background: Background: Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.

UniProt: P07374