XDH antibody (Biotin)

Catalog No. ABIN1607817

Product Details anti-XDH Antibody (Biotin)

Target: XDH (Xanthine Dehydrogenase (XDH))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Xanthine Oxidase from other sources is unknown.

Purity: Anti-Xanthine Oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Endotoxin Level: Low Endotoxin : No

Immunogen: Xanthine Oxidase [Bovine Buttermilk]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Xanthine Oxidase antibody has been assayed against 1.0 µg of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10.000 to 1:40.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: Xanthine Oxidase (XDH Products)

Synonyms: XO antibody, XOR antibody, Xor antibody, Xox-1 antibody, Xox1 antibody, XDH antibody, im:7146167 antibody, DDBDRAFT_0219626 antibody, DDBDRAFT_0230176 antibody, DDB_0219626 antibody, DDB_0230176 antibody, xanthine dehydrogenase antibody, xanthine dehydrogenase/oxidase antibody, Xanthine dehydrogenase antibody, XDH antibody, Xdh antibody, xdh antibody, LOC576712 antibody, Trebr_1754 antibody

Background: Anti-Xanthine Oxidase recognizes xanthine oxidase, an oxidoreductase. Xanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid. Both of these reactions also generate the reactive oxygen species hydrogen peroxide. In addition, xanthine oxidase is involved in the catabolism of purines and metabolism of caffeine. Clinically, xanthine oxidase has been linked to gout, a condition in which uric acid accumulates in the kidneys and joints. Anti-Xanthine Oxidase antibody is ideal for investigators interested in Metabolism and Signal Transduction research. Synonyms: Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

Gene ID: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity