RNASE1 antibody (Biotin)

Catalog No. ABIN1607821

Product Details anti-RNASE1 Antibody (Biotin)

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNASE1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Cross-Reactivity (Details): Cross reactivity against Ribonuclease A from other sources may occur but have not been specifically determined.

Purity: Anti-Ribonuclease A antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas].

Endotoxin Level: Low Endotoxin : No

Immunogen: Ribonuclease A [Bovine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Ribonuclease A antibody has been assayed against 1.0 µg of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:13.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for RNASE1

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Alternative Name: Ribonuclease A (RNASE1 Products)

Synonyms: RNASE1 antibody, RNS1 antibody, ATRNS1 antibody, RIBONUCLEASE 1 antibody, T17M13.16 antibody, T17M13_16 antibody, ribonuclease 1 antibody, RIB1 antibody, AI574248 antibody, Rib-1 antibody, Rib1 antibody, SRN antibody, ribonuclease A family member 1, pancreatic antibody, ribonuclease pancreatic-like antibody, ribonuclease A family member k6 antibody, Ribonuclease 1 antibody, ribonuclease 1 antibody, ribonuclease, RNase A family, 1 (pancreatic) antibody, ribonuclease pancreatic antibody, RNASE1 antibody, LOC475395 antibody, RNASE6 antibody, Rnase1 antibody, RNS1 antibody, LOC101113761 antibody

Background: Anti-Ribonuclease A recognizes ribonuclease, an endoribonuclease that catalyzes the cleavage of the phosphodiester bond between 5'-ribose of a nucleotide and the phosphate group of a 3'-ribose of a pyrimidine nucleotide. Able to survive acidic and near boiling conditions, RNase A is highly stable. RNase A and its homologs have been demonstrated to have cytotoxic and cytostatic effects. Synonyms: Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody

Gene ID: 282340

UniProt: P61823