H2AFX antibody (pSer139)

Catalog No. ABIN1607875

Supplier Product No.: 600-401-h36

Product Details anti-H2AFX Antibody

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Binding Specificity: pSer139

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-h36

Supplier: Rockland

Purpose: H2AX phospho S139 Antibody

Cross-Reactivity (Details): H2AX pS139 is directed against the phosphorylated form of human H2AX protein at the pS139 residue.

Characteristics: Synonyms: rabbit anti-H2AX pS139 antibody, rabbit anti-Histone H2AX pS139 antibody, H2AFX antibody, H2a/x, H2A.x, H2AX, histone H2A, gamma-H2AX, H2A histone family member X antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-H2AX pS139 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the C-terminal region containing serine 139 of human H2AX protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Application Note: Anti-H2AX pS139 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to phosphorylated H2AX protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Histone H2A.X (phospho S139) antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-H2AFX antibody (ABIN1607875)

Rivera-Casas, Gonzalez-Romero, Garduño, Cheema, Ausio, Eirin-Lopez: "Molecular and Biochemical Methods Useful for the Epigenetic Characterization of Chromatin-Associated Proteins in Bivalve Molluscs." in: Frontiers in physiology, Vol. 8, pp. 490, (2017) (PubMed).

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX (H2AFX Products)

Background: Background: Histone H2A.X (phospho S139) antibody is ideal for western blotting and ELISA. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA is wrapped around histone-groups, consisting of the core histones H2A, H2B, H3 and H4. As a reaction on DNA Double-strand breaks (DSB) H2AX becomes phosphorylated on serine 139, called gamma-H2AX. ATM, ATR and PRKDCs, kinases of the PI3-family, are responsible for this phosphorylation. The modification can happen accidentally during replication fork collapse, exogenous genotoxic agents, may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes, in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination. Mutagenesis experiments have shown that the modification is necessary for the proper formation of ionizing radiation induced foci in response to double strand breaks, but is not required for the recruitment of proteins to the site of DSBs. Gamma-H2AX is a sensitive target for looking at DSBs in cells. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. The role of the phosphorylated form of the histone in DNA repair is under. Anti-H2AX pS139 is ideal for researched interested in Histones, DNA Damage and Repair, and Epigenetics.

Gene ID: 3014

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus