ARHGAP22 antibody (pSer397)

Catalog No. ABIN1607879

Supplier Product No.: 600-401-j12

The Rabbit Polyclonal anti-ARHGAP22 antibody has been validated for WB, ELISA, IHC and FM. It is suitable to detect ARHGAP22 in samples from Human, Mouse and Rat.

Quick Overview for ARHGAP22 antibody (pSer397) (ABIN1607879)

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGAP22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-ARHGAP22 Antibody

Binding Specificity: pSer397

Supplier Product No.: 600-401-j12

Supplier: Rockland

Purpose: Arhgap22 phospho S397 Antibody

Cross-Reactivity (Details): This antibody is specific for phosphorylated ARHGAP22 at Serine 397.

Characteristics: Synonyms: rabbit anti-ARHGAP pS397 antibody, ARHGAP 22, ARHGAP-22, Rho-type GTPase-activating protein 22, RHOGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22

Purification: Anti-ARHGAP22 pS397 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-ARHGAP22 pS397 antibody is tested for Western Blot and Immunostaining and useful for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.07 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for ARHGAP22

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Alternative Name: Arhgap22

Background: Background: ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser397] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research.

Gene ID: 239027

NCBI Accession: NP_722495

UniProt: Q8BL80