APP antibody (N-Term)

Catalog No. ABIN1607881

Supplier Product No.: 600-401-j14

This Rabbit Polyclonal antibody specifically detects APP in WB, ELISA, IHC, IF, FM, DB and MA. It exhibits reactivity toward Human.

Quick Overview for APP antibody (N-Term) (ABIN1607881)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Dot Blot (DB), Multiplex Assay (MA)

Product Details anti-APP Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-j14

Supplier: Rockland

Purpose: Beta Amyloid pyro E3 Antibody

Cross-Reactivity (Details): This antibody contains no reactivity towards the 1-42 ABeta peptide.

Characteristics: Synonyms: rabbit anti-Beta Amyloid pyro E3 Antibody, ß-amyloid pyro E3, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI

Purification: Anti-Beta Amyloid pyro Glu3 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Beta Amyloid [Pyro Glu3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 3-pyro E start point of human beta Amyloid.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-Beta Amyloid pyro Glu3 has been tested in dot blot, ELISA, Western Blot, and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background: Background: The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. Beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. Beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. Beta Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimer's disease. Anti-beta Amyloid [Pyro Glu11] Antibody is ideal for researchers interested in Alzheimer's Research, Cell Cycle and Replication, and Neuroscience research.

Gene ID: 351

NCBI Accession: NP_000475

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour