HDAC2 antibody (C-Term)

Catalog No. ABIN1607885

Supplier Product No.: 600-401-j18

The Rabbit Polyclonal anti-HDAC2 antibody has been validated for WB, IHC, ELISA and FM. It is suitable to detect HDAC2 in samples from Human, Mouse, Rat and Primate.

Quick Overview for HDAC2 antibody (C-Term) (ABIN1607885)

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Reactivity: Human, Mouse, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Product Details anti-HDAC2 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-j18

Supplier: Rockland

Purpose: HDAC2 (near C-terminus) Antibody

Cross-Reactivity (Details): This antibody is specific towards HDAC2.

Characteristics: Synonyms: rabbit anti-HDAC-2 antibody, HDAC2, HDAC 2, HD 2 antibody, histone deacetylase 2 antibody, Histone deacetylase-2, HD2 antibody

Purification: Anti-HDAC2 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: HDAC2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near C-terminal region of human HDAC2.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-HDAC2 antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~59 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for HDAC2

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Alternative Name: HDAC2

Background: Background: Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human. Anti-HDAC2 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Chromatin Research, Epigenetics, Histone Deacetylases, and Stem Cell Markers research.

Gene ID: 3066

NCBI Accession: NP_001518

UniProt: Q92769

Pathways: Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection