HDAC4 antibody (AA 600-650)

Catalog No. ABIN1607887

Supplier Product No.: 600-401-j20

This Rabbit Polyclonal antibody specifically detects HDAC4 in WB, ELISA, IHC and FM. It exhibits reactivity toward Human, Mouse, Rat and Primate.

Quick Overview for HDAC4 antibody (AA 600-650) (ABIN1607887)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivity: Human, Mouse, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-HDAC4 Antibody

Binding Specificity: AA 600-650

Supplier Product No.: 600-401-j20

Supplier: Rockland

Purpose: HDAC4 (internal) Antibody

Cross-Reactivity (Details): This antibody is specific towards HDAC4.

Characteristics: Synonyms: rabbit anti-HDAC-4 antibody, HDAC4, HDAC 4, HD 4 antibody, histone deacetylase 4 antibody, Histone deacetylase-4, HD4 antibody

Purification: Anti-HDAC4 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1 mg/mL

Application Note: Anti-HDAC4 antibody has been tested in Western Blot and is useful for ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~140 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 mg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: HDAC4

Background: Background: HDAC4 is a 140 kD class II histone deacetylase similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus. Anti-HDAC4 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.

Gene ID: 9759

NCBI Accession: NP_006028

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process